Mihai Macovei (left calf tear), Mihai Mureșan (shoulder dislocation) and Paul Popoaia (subacromial bursitis and supraspinous tendinitis) have been replaced by Andre Gorin, Lama Sioeli and Taliauli Sikuea.
Marius Iftimiciuc and Florin Surugiu are in rehab, set to return to training on 5 September.
Cristi Chirica will captain Romania at his first Rugby World Cup with the squad made up of 19 forwards and 14 backs.
Alexandru Savin (CSA Steaua)
Robert Irimescu (CSM Știința Baia Mare)
Adrian Motoc (Biarritz Olympique)
Vlad Neculau (SCM USV Timișoara)
Florian Roșu (CSM Știința Baia Mare)
Dragoș Ser (CSA Steaua)
Damian Strătilă (CSA Steaua)
Gabriel Rupanu (SCM USV Timișoara)
Florin Surugiu (CSA Steaua)
Tudor Boldor (CS Dinamo)
Jason Tomane (CSM Știința Baia Mare)
Nicolas Onuțu (CS Vienne)
Marius Simionescu (SCM USV Timișoara)
Tangimana Fonovai (CSA Steaua)
Hinckley Vaovasa (CSA Steaua)
Romania will play pool games against Ireland (9 September), South Africa (17 September), Scotland (30 September) and Tonga (8 October).
Players withdrawn through injury: Mihai Macovei, Mihai Mureșan, Paul Popoaia