Jonny May (Gloucester Rugby) and Alex Mitchell (Northampton Saints) are included in the squad replacing Jack van Poortvliet (Leicester Tigers) and Anthony Watson.
Owen Farrell will captain England in his third Rugby World Cup with Ellis Genge and Courtney Lawes named as vice captains.
The squad is made up of 19 forwards and 14 backs. Seventeen players have previous Rugby World Cup experience with three - Dan Cole, Courtney Lawes and Ben Youngs - set to play in their fourth RWC tournament.
(changes to original announcement appear in bold)
Forwards
Bevan Rodd (Sale Sharks, 3 caps)
Kyle Sinckler (Bristol Bears, 62 caps)*
Will Stuart (Bath Rugby, 26 caps)
Jack Walker (Harlequins, 3 caps)
David Ribbans (Toulon, 6 caps)
Tom Curry (Sale Sharks, 45 caps)*
Ben Earl (Saracens, 15 caps)
Courtney Lawes (Northampton Saints, 97 caps)***
Lewis Ludlam (Northampton Saints, 20 caps)*
Billy Vunipola (Saracens, 68 caps) **
Jack Willis (Toulouse, 10 caps)
Backs
Ben Youngs (Leicester Tigers, 122 caps)***
Marcus Smith (Harlequins, 22 caps)
Manu Tuilagi (Sale Sharks, 51 caps)**
Henry Arundell (Racing 92, 7 caps)
Elliot Daly (Saracens, 57 caps)*
George Ford (Sale Sharks, 82 caps)**
Max Malins (Bristol Bears, 19 caps)
Jonny May (Gloucester Rugby, 73 caps **)
Freddie Steward (Leicester Tigers, 23 caps)
*denotes number of Rugby World Cup tournaments played
Players replaced from original squad of 33: Jack van Poortvliet, Anthony Watson