Colombia and Brazil will compete in a two-legged play-off at Estadio Cincuentenari in Medellin on 5 and 9 July to decide which side will secure their place in WXV 3, the third tier of World Rugby’s new annual global competition.

WXV 3 is scheduled to take place across the weekends of 14, 21 and 28 October.

The teams last came face-to-face on 11 June when Las Tucanes won a closely contested match, 18-15 at the Americas Rugby Trophy. Colombia have won all four matches between the sides to date.

Brazil have announced a 30-strong squad that will take part in a training camp between 26-30 June in São Paulo, in preparation for their matches against Colombia.

As Yaras have added three sevens players to the squad that played in the Americas Rugby Trophy, against Colombia and USA U23, on home soil earlier this month.

Lissete Martínez, Colombia’s head coach, said: "The confrontation against Brazil is very important, we know it's going to be a very difficult game. The last few matches there has been very little difference, they have been working and so have we.

“We will improve the aspects that we saw did not turn out so well in the Trophy, focusing on improving defence, winning and keeping the ball."

Brazil’s head coach, AK Southey, added: “For me it is one of the biggest historical matches for Brazil. We are yet to beat Colombia but we came close at the Americas Trophy.

“We are not just playing for our team, we are playing for every girl playing rugby in Brazil. For us to play for them and have the support of them is important.

“For every player that attend our camps, it was hard work and it was not easy. None of us just play rugby, we all have wider commitments, so for us, it is historical for us to go there. We have one aim and that’s to qualify for WXV and that’s what we are working towards.”

Fiji, Ireland, Kazakhstan and Kenya have already confirmed their places in WXV 3 via regional qualification tournaments. The two remaining spots will be filled by the winner of the two-legged play-off between Colombia and Brazil and the loser of the play-off between Italy and Spain, which will be played on 22 July in Piacenza, Italy.